Research

Boronic Acid: A Bio-Inspired Strategy to Increase the Sensitivity and Selectivity of Fluorescent NADH Probe

Boronic Acid: A Bio-Inspired Strategy to Increase the Sensitivity and Selectivity of Fluorescent NADH Probe

Fluorescent probes have been widely used in the molecular recognition events in biological systems; however, it remains challenging to design small-molecule fluorescent probes sensitively and selectively sensing biomolecules with complex structures. Inspired by the enzyme-catalyzed reaction between biomolecule and probe, Prof. Chang and collaborators present a novel combination-reaction two-step sensing strategy to improve sensitivity and selectivity. Based on this strategy, they successfully prepared a boronic acid based fluorescent NADH (reduced nicotinamide adenine dinucleotide) probe and evaluate NADH levels in live cell imaging.
J. Am. Chem. Soc., 2016, 138 (33), pp 10394–10397
Link

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