Thorsten WOHLAND

Professor

Postdoctoral., Stanford University (USA); ; PhD, Swiss Federel Institute of Technology at Lausanne (EPFL, Switzerland); Dipl. Phys., University of Heidelberg, Germany.

Contact Information:

Office: S1A-02-12

Tel: (65)-6516-1248

Fax: (65)-6776-7882

Email: twohland@nus.edu.sg

Research

ORCID: 0000-0002-0148-4321
ResearcherID: H-8285-2012

Research Interests

My group works on the development of new fluorescence spectroscopy techniques for the measurement of biomolecular interactions in cells, tissues, and organisms. Specific foci are:

Techniques: Single wavelength excitation fluorescence cross-correlation spectroscopy (SW-FCCS) and imaging fluorescence correlation spectroscopy (ITIR-FCS, SPIM-FCS).
Biological systems: Transmembrane protein receptors and peptides and their interaction with membranes, measurement of biomolecular actions and interactions in living cells and organisms.

Research Highlight

In recent years our group focused on developing image based fluorescence tech-niques in which the signal at each pixel of a camera is evaluated to determine sample structure, dynamics, and interactions and single molecule sensitivity. We have implemented these techniques on total internal reflection (TIR) and single plane illumination microscopes (SPIM). Abbreviations: FCS – fluorescence correlation spectroscopy; FCCS – fluorescence cross-correlation spectroscopy; N&B – number and brightness analysis; FRAP – Fluorescence Recovery After Photobleaching; SRRF – super-resolution radial fluctuation; SPT – single particle tracking; spFRET – single particle Förster resonance energy transfer.

Representative Publications

Tang, W. H.; Sim, S. R.; Aik, Y. K.; Nelanuthala, A. V. S.; Athilingam, T.; Röllin, A.; Wohland, T., Deep learning reduces data requirements and allows real-time measurements in Imaging FCS. Biophys. J. 2024, in press. https://www.biorxiv.org/content/10.1101/2023.08.07.552352v1.
Sankaran, J.; Wohland, T., Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications. Commun. Biol. 2023, 6, 699, DOI: doi.org/10.1038/s42003-023-05069-6.
Balasubramanian, H.; Sankaran, J.; Pandey, S.; Goh, C. J. H.; Wohland, T., The dependence of EGFR oligomerization on environment and structure: A camera-based N&B study. Biophys. J. 2022, DOI: doi.org/10.1016/j.bpj.2022.11.003.
Tantirimudalige, S. N.; Raghuvamsi, P. V.; Sharma, K. K.; Bao, J. C. W.; Anand, G. S.; Wohland, T., The ganglioside GM1a functions as a coreceptor/attachment factor for dengue virus during infection. J. Biol. Chem. 2022, DOI: doi.org/10.1016/j.jbc.2022.102570.
Aik, D. Y. K.; Wohland, T., Microscope alignment using real-time Imaging FCS. Biophys. J. 2022, DOI: doi.org/10.1016/j.bpj.2022.06.009.
Gupta, A.; Lu, D.; Balasubramanian, H.; Chi, Z.; Wohland, T., Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes. J. Lipid Res. 2022, DOI: doi.org/10.1016/j.jlr.2022.100220.
Yong, X. E.; Palur, V. R.; Anand, G. S.; Wohland, T.; Sharma, K. K., Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences. Nucleic Acids Res. 2021, DOI: 10.1093/nar/gkab379.
Sankaran, J.; Balasubramanian, H.; Tang, W. H.; Ng, X. W.; Röllin, A.; Wohland, T., Simultaneous Spatiotemporal Super-Resolution and Multi-Parametric Fluorescence Microscopy. Nat. Commun. 2021, DOI: 10.1038/s41467-021-22002-9.
Koh, A.; Tao, S.; Goh, Y. J.; Chaganty, V.; See, K.; Purushothaman, K.; Orbán, L.; Mathuru, A. S.; Wohland, T.; Winkler, C., A Neurexin2aa deficiency results in axon pathfinding defects and increased anxiety in zebrafish. Hum. Mol. Genet. 2021, DOI: 10.1093/hmg /ddaa260.
Veerapathiran, S.; The, C.; Zhu, S.; Kartigayen, I.; Korzh, V.; Matsudaira, P. T.; Wohland, T., Wnt3 distribution in the zebrafish brain is determined by expression, diffusion and multiple molecular interactions. eLife 2020, DOI: 10.7554/eLife.59489.

Thorsten WOHLAND