Thorsten WOHLAND

Thorsten WOHLAND

Professor

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Research

ORCID:  0000-0002-0148-4321
ResearcherID: H-8285-2012

 

Research Interests

My group works on the development of new fluorescence spectroscopy techniques for the measurement of biomolecular interactions in cells, tissues, and organisms. Specific foci are:

• Techniques: Imaging fluorescence correlation and cross-correlation spectroscopy (ITIR-FCS/FCCS, SPIM-FCS/FCCS).
• Data Analysis: Development of statistical methods and deep learning to treat single molecule fluorescence data.
• Biological systems: Transmembrane protein receptors and peptides and their interaction with membranes, measurement of biomolecular actions and interactions in living cells and organisms, viral infection mechanisms, and biofilm characteristics.

 

Research Highlight

In recent years our group focused on developing image based fluorescence techniques in which the signal at each pixel of a camera is evaluated to determine sample structure, dynamics, and interactions and single molecule sensitivity with millisecond time and 100 nm spatial resolution. We have implemented these techniques on total internal reflection (TIR) and single plane illumination microscopes (SPIM). We established new statistical and deep learning techniques for single molecule fluorescence data analysis and we applied our new techniques to a range of biological problems ranging from protein dynamics, viral infection mechanisms, and biofilm to morphogenesis in developmental biology.

 

Representative Publications   

• Wohland, T.; Sim, S. R.; Demoustier, M.; Pandey, S.; Kulkarni, R.; Aik, D. Y. K. FCS Videos: Fluorescence Correlation Spectroscopy in Space and Time. Biochim. Biophys. Acta 2024, In Press. DOI: 10.1016/j.bbagen.2024.130716.
• Zhu, S.; Loo, Y. T.; Veerapathiran, S.; Loo, T. Y. J.; Tran, B. N.; Teh, C.; Zhong, J.; Matsudaira, P.; Saunders, T. E.; Wohland, T. Receptor Binding and Tortuosity Explain Morphogen Local-to-Global Diffusion Coefficient Transition. Biophys. J. 2024, In Press. DOI: 10.1016/j.bpj.2024.07.024.
• Athilingam, T.; Nelanuthala, A. V. S.; Breen, C.; Karedla, N.; Fritzsche, M.; Wohland, T.; Saunders, T. E. Long-Range Formation of the Bicoid Gradient Requires Multiple Dynamic Modes That Spatially Vary Across the Embryo. Development 2024, In Press. DOI: 10.1242/dev.202128.
• Tang, W. H.; Sim, S. R.; Aik, D. Y. K.; Nelanuthala, A. V. S.; Athilingam, T.; Röllin, A.; Wohland, T. Deep Learning Reduces Data Requirements and Allows Real-Time Measurements in Imaging FCS. Biophys. J. 2023, 122 (11), 3403. DOI: 10.1016/j.bpj.2023.11.3403.
• Sankaran, J.; Wohland, T. Current Capabilities and Future Perspectives of FCS: Super-Resolution Microscopy, Machine Learning, and In Vivo Applications. Commun. Biol. 2023, 6, 5069. DOI: 10.1038/s42003-023-05069-6.
• Balasubramanian, H.; Sankaran, J.; Pandey, S.; Goh, C. J. H.; Wohland, T. The Dependence of EGFR Oligomerization on Environment and Structure: A Camera-Based N&B Study. Biophys. J. 2022, 121 (11), 003. DOI: 10.1016/j.bpj.2022.11.003.
• Tantirimudalige, S. N.; Raghuvamsi, P. V.; Sharma, K. K.; Bao, J. C. W.; Anand, G. S.; Wohland, T. The Ganglioside GM1a Functions as a Coreceptor/Attachment Factor for Dengue Virus During Infection. J. Biol. Chem. 2022, 298 (9), 102570. DOI: 10.1016/j.jbc.2022.102570.
• Aik, D. Y. K.; Wohland, T. Microscope Alignment Using Real-Time Imaging FCS. Biophys. J. 2022, 121 (6), 009. DOI: 10.1016/j.bpj.2022.06.009.
• Yong, X. E.; Palur, V. R.; Anand, G. S.; Wohland, T.; Sharma, K. K. Dengue Virus 2 Capsid Protein Chaperones the Strand Displacement of 5′-3′ Cyclization Sequences. Nucleic Acids Res. 2021, 49 (11), 379. DOI: 10.1093/nar/gkab379.
• Sankaran, J.; Balasubramanian, H.; Tang, W. H.; Ng, X. W.; Röllin, A.; Wohland, T. Simultaneous Spatiotemporal Super-Resolution and Multi-Parametric Fluorescence Microscopy. Nat. Commun. 2021, 12, 22002. DOI: 10.1038/s41467-021-22002-9.