Professor LEE Hian Kee

B.Sc., 1978, University of Canterbury; Ph.D., 1982, University of Canterbury; Special Volunteer, 1991/92, National Institutes of Health, Bethesda, USA

Contact Information

Department of Chemistry, NUS 
3 Science Drive 3 
Singapore 117543 
Office: S5-02-12
Tel: (65)-6516-2995
Fax: (65)-6779-1691
Email: chmleehk@nus.edu.sg


Research Interests

Analytical Chemistry


Our research interests are focused on capillary electrophoresis, liquid chromatography (LC, including nano-LC), and gas chromatography-mass spectrometry. We have a particular interest in developing extraction procedures like cartridge- and disk-based solid-phase extraction, and solventless and solvent-minimised extractionprocedures such as solid- and liquid-phase microextraction (see figure below). Other interests in extraction are on microwave-assisted extraction, and super- and subcritical fluid extraction. All these techniques are mainly applied to environmental analyses although we employ them for the analysis of molecules of biological importance as well. Some analytes of current interest include environmental carcinogens such as heterocyclic aromatic amines, polycyclic aromatic hydrocarbons, pesticides, phenols, organometals, inorganic anions, endocrine disruptors and biological compounds like steroids, chiral drugs, etc. 

We are additionally interested in the LC retention behaviour of transition metal complexes important in catalysis. We are developing new inorganic materials for use in inorganic synthetic and industrial catalytic reactions, including the chemical detoxification of environmental pollutants (polychlorinated biphenyls, etc).



Representative Publications

  • Zhang, H. & Lee, H.K. Plunger-in-Needle Solid-Phase Microextraction with Graphene-Based Sol-Gel Coating as Sorbent for Determination of Polybrominated Diphenyl Ethers. J. Chromatogr. A (2011) 1218, 4509-4516
  • Lee, J. & Lee, H.K. Fully-Automated Dynamic In-Syringe Liquid-Phase Microextraction and On-Column Derivatization of Carbamate Pesticides with Gas Chromatography/Mass Spectrometric Analysis. Anal. Chem. (2011) 83, 6856-6861
  • Shi Z.-G. & Lee, H.K. Dispersive Liquid-Liquid Microextraction Coupled with Dispersive Micro-Solid-Phase Extraction for the Fast Determination of Polycyclic Aromatic Hydrocarbons in Environmental Water Samples. Anal. Chem. (2010) 82, 1540-1545
  • Basheer, C., Alnedhary, A.A., Rao, B.S.M., Valiyavettil, S. & Lee, H.K. Development and Application of Porous Membrane-Protected Carbon Nanotube Micro-Solid-Phase Extraction Combined with Gas Chromatography/Mass Spectrometry. Anal. Chem. (2006) 78, 2853-2858
  • X. Jiang, & H.K. Lee. Dynamic Liquid-Liquid-Liquid Microextraction with Automated Movement of the Acceptor Phase. Anal. Chem., 77, 1689-1695 (2005)
  • J. Zhang, T. Su, & H.K. Lee. Headspace Water-Based Liquid-Phase Microextraction. Anal. Chem., 77, 1988-1992 (2005)
  • C. Basheer, S. Sindhu, H.K. Lee & S. Valiyaveettil. Development and Application of a Simple Capillary-Microreactor for Oxidation of Glucose with a Porous Gold Catalyst. Chem. Comm., 409-410 (2005)
  • Jiang, X.M. & Lee, H.K. Solvent Bar Microextraction. Anal. Chem. (2004) 76, 5591-5596
  • C.H. Tu, L. Zhu, C.H. Ang, & H.K. Lee. Effect of NaOH on Large Volume Sample Stacking of Haloacetic Acids in Capillary Zone Electrophoresis with a Low pH Buffer. Electrophoresis, 24, 2188-2192 (2003)
  • G. Shen & H.K. Lee. Headspace Liquid-Phase Microextraction of Chlorobenzenes in Soil with Gas Chromatography-Electron Capture Detection. Anal. Chem., 75, 98-103 (2003)
  • L. Zhu, C.H. Tu & H.K. Lee. On-Line Concentration of Acidic Compounds by Anion-Selective Exhaustive Injection-Sweeping-Micellar Electrokinetic Chromatography. Anal. Chem., 74, 5820-5825 (2002)
  • Shen, G. & Lee, H.K. Hollow Fiber-Protected Liquid-Phase Microextraction of Triazine Herbicides. Anal. Chem. (2002) 74, 648-654
  • He, Y. & Lee, H.K. Large Volume Sample Stacking in Acidic Buffer for Fast and Sensitive Analysis of Small Organic and Inorganic Anions by Capillary Electrophoresis. Anal. Chem. (1999) 71, 995-1001
  • Wang Y., Kwok, Y.C., He, Y. & Lee, H.K. Application of Dynamic Liquid-Phase Microextraction to the Analysis of Chlorobenzenes in Water Using a Conventional Microsyringe. Anal. Chem. (1998) 70, 4610-4614
  • He, Y. & Lee, H.K. Liquid-Phase Microextraction in a Single Drop of Organic Solvent by Using a Conventional Microsyringe. Anal. Chem. (1997) 69, 4634-4640